| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Bacillus subtilis | ACGGCTGCGAAAACAACAC | AACAGGCCTGCGTTCACA | 59.94 | 59.81 | 158 | 93 |
100.00%
|
100.00%
|
| Primer 22 | Bacillus subtilis | TCACCGGACGCCTGATTTC | AACAGGCCTGCGTTCACA | 60.08 | 59.81 | 264 | 93 |
100.00%
|
100.00%
|
| Primer 23 | Bacillus subtilis | GGATGACGGCTGCGAAAAC | AACAGGCCTGCGTTCACA | 59.87 | 59.81 | 163 | 93 |
100.00%
|
100.00%
|
| Primer 24 | Bacillus subtilis | GCACAGCATGCGAAACAGT | TGGCGTTCCCGTGATGATC | 59.72 | 60.15 | 220 | 93 |
100.00%
|
100.00%
|
| Primer 25 | Bacillus subtilis | ACGGAGCACATCGCATGT | GATTCGGCTGCTTGGACAC | 59.73 | 59.20 | 212 | 92 |
100.00%
|
100.00%
|
| Primer 26 | Bacillus subtilis | AGCACAAGGGATGGTCGTG | ACCCCTTCAGCCGTATGTG | 60.00 | 59.40 | 261 | 92 |
100.00%
|
100.00%
|
| Primer 27 | Bacillus subtilis | GCACAGCATGCGAAACAGT | TGGCGTTCCCGTGATGAT | 59.72 | 59.01 | 220 | 91 |
100.00%
|
50.00%
|
| Primer 28 | Bacillus subtilis | CCGCTCTGCACTTTCGGTA | TGACAGGCAAACACGGCA | 60.08 | 60.12 | 151 | 89 |
100.00%
|
100.00%
|
| Primer 29 | Bacillus subtilis | ACCGGACGCCTGATTTCA | AACAGGCCTGCGTTCACA | 58.93 | 59.81 | 262 | 88 |
100.00%
|
100.00%
|
| Primer 30 | Bacillus subtilis | TGCGCACCTTGCAAGACT | AGCCGTCTTTATGGTCAGGC | 60.20 | 60.11 | 227 | 88 |
100.00%
|
100.00%
|